Originally published in the Feb. 19, 2014, issue
Visit with a
Georgia veterinarian in this monthly feature. This edition comes from Dr. John
K. Bernard, animal and dairy science professor on the UGA-Tifton campus.
Blood sample analysis is the traditional way
of evaluating a dairy cow’s calcium status. The sample is analyzed for total
calcium concentrations. This is calcium that meets three specifications: bound
to plasma protein; in an ionic form associated with low molecular weight
compounds; and free ions. Metabolic functions such as muscle contraction, blood
clotting and bone formation is supported by the free ion form of calcium.
Ionized calcium measures these free calcium
ions, which are readily available for the aforementioned metabolic functions.
This provides a better estimate of true calcium status. The proportion of total
calcium that is ionized is not constant, and changes with lactation stages.
Occasionally, some calcium concentrations indicate the cow is not ill, but
looking at ionized calcium concentrations confirm she is in a state of clinical
hypocalcemia.
Ionized calcium concentrations of less than
one millimol per liter are considered indicators of subclinical milk fever. Both
ionized calcium and magnesium levels can be used to evaluate nutrition in
close-up dry and transition cow nutrition, as well as diagnose milk fever and
grass tetany that occur around parturition or early lactation.
Recently, an electrode-based method was
developed for rapid measurement of ionized calcium. This method is both
economical and available to researchers and producers, and a unit is located at
the University of Georgia Veterinary Diagnostic and Investigational Laboratory
in Tifton, Ga.
Samples of ionized calcium and magnesium should
be collected in a green top (Lithium Heparin) with a separator gel Vacutainer
tube, or a “tiger-top” gel-separator tube. The sample should be allowed to
clot, centrifuged to separate the plasma or serum from the blood clot and
refrigerated immediately. The frozen sample should be packaged with an ice pack
and shipped for overnight delivery to the laboratory for analysis. The pH of
the sample is measured and used to calculate the final result.
Do not remove the cap from the collection
tube. Exposing samples to air or long storage can change blood pH, so it is
important to collect and transmit and process samples quickly in a laboratory
to obtain valid results.
For
questions about submitting ionized calcium and magnesium samples, contact Anita
Merrill at 229-386-3340.